Published at: Sat, 27 Feb 2021 11:49 AM AEDT as final startlist. Changes may still occur prior to event.
| Category | Number | Participant | Club/Team |
|---|---|---|---|
| 0:00:00 | 3 | O'ROURKE, Glen | Footscray Cycling Club |
| 0:00:00 | 184 | OWEN, David | Bendigo & District Cycling Club |
| 0:00:00 | 20 | SEDDON, Mark | Northern Cycling Incorporated/Seymour |
| 0:04:00 | 38 | MCDONOUGH, Tom | Northern Cycling |
| 0:04:00 | 26 | MOGAVERO, John | Northern Vets Cycling Club |
| 0:04:00 | 6 | ROMANO, Emilio | Northern Cycling |
| 0:04:00 | 200 | TROVATELLO, Keith | Hawthorn Cycling Club |
| 0:07:00 | 51 | FARRAN, Tom | Northern Cycling club |
| 0:07:00 | 49 | FOSTER, Cristine | Northern Cycling |
| 0:07:00 | 84 | HODGES, Terrance | Northern Cycling Club Inc |
| 0:07:00 | 91 | HOLLIBONE, Terry | Northern Cycling |
| 0:07:00 | 99 | NOMIKOS, Nick | Northern Cycling |
| 0:09:30 | 116 | ANDERSON, Tim | Northern Cycling |
| 0:09:30 | 43 | KNIGHT, Pete | Northern Cycling |
| 0:09:30 | 95 | LAVENDER, Michael | Northern Cycling club |
| 0:09:30 | 94 | SAID, Noel | Seymour Broadford Cycling Club |
| 0:11:00 | 74 | EDELE, Mark | Northern Veterans Cycling Club |
| 0:11:00 | 139 | GOODROPE, George | Northern Cycling |
| 0:11:00 | 60 | HARTMAN, Michael | Northern Cycling club |
| 0:11:00 | 183 | SMITH, Michael | Northern Cycling Incorporated |
| 0:13:30 | 79 | RIM, Trevor | Northern Cycling |
| 0:13:30 | 182 | SANTALUCIA, Frank | Brunswick Cycling Club |
| 0:13:30 | 96 | SAUNDERS, Stephen | Northern Cycling Incorporated |
| 0:13:30 | 138 | SHORT, John | Northern Cycling |
| 0:16:00 | 112 | BOL, Simon | Northern Cycling Incorporated |
| 0:16:00 | 180 | BONNETT, David | Brunswick Cycling Club |
| 0:16:00 | 108 | CARUANA, Joe | Footscray Cycling Club |
| 0:16:00 | 152 | MARKS, Brian | Macedon Ranges Cycling Club |
| 0:21:00 | 181 | BEASLEY, Paul | Footscray Cycling Club |
| 0:21:00 | 133 | GHERXI, Tony | Northern Cycling Club Inc |
| 0:21:00 | 144 | HERBEC, Henryk | Northern Cycling Incorporated |
| 0:21:00 | 141 | SINNI, Vince | Northern Cycling |
| 0:21:00 | 149 | SMYRK, Lindsay | Northern Cycling |
| 0:28:00 | 148 | READ, Jo | Northern Cycling |
| 0:28:00 | 178 | SIMPSON, Tanya | Northern Cycling Club |
| 0:28:00 | 1 | WATT, Allison | Northern Cycling |
| 0:42:00 | 169 | KELLY, Meredith | Northern Veterans Cycling Club |